Brook is known for his strange personality, being a combination of a gentlemanly persona and a perverted sort. He speaks in a dignified, educated manner at most times, but is known to have amazingly bad manners (burping and farting at dinner, casually asking to see womens' panties, picking at other peoples' food, etc).
Brook is also known to adore bone puns. He takes any chance he can to make a comment about his skeletal nature, almost inevitably following with "Yoho! Skull joke!!" Example: "I was so shocked, my eyes nearly popped out of my head! BUT I HAVE NO EYES!!"
Brook has a distinct laugh, always known to give off a jolly "Yohoho!"
Brook carries a shikomizue (Japanese cane sword), and is extremely skilled in its use. He can also combine his shikomizue and violin to create sound-based attacks. He was once a fighter in the surprise-attack squadron of an unknown kingdom. In addition to his sword skill, Brook's lightweight skeleton body gives him great speed and the ability to jump extraordinarily high. His body is so light that he is even capable of running on water, however, Brook is still powerless if submerged in water, like all Devil Fruit users. Brook is also an excellent musician, displaying the ability to play any instrument at a highly skilled level. He's even capable of playing songs that drive anyone who hears them to sleep, which proves to be useful in combat. Brook claims he can heal from most fractures to his body by drinking milk. His head can also be opened up and used as a storage device, which is currently being used to store a Tone Dial containing the last song of the Rumba Pirates. Brook uses an unnamed fencing sword style and is actually quite a skilled fencer and can use quick stabs because of his skeleton anatomy.
One of his signature attacks is the "Arrow Notch Strike", a sword technique so fast that the actual strike is not seen, and the recipient is unaware they have been struck until a significant amount of time after the attack (usually a few seconds, about the amount of time it takes to resheath his sword).
During his time on Thriller Bark, Brook developed a reputation among the inhabitants as a mysterious warrior who would sing a haunting tune just before he struck down a squad of zombies in a flash. This turned out to be because Brook himself was afraid of the horrible place, and sought to calm himself by humming or singing as he went. He also used his superior speed to mow down the opponents, mostly so that he could not scare himself with their appearance.
While dead and seemingly able to last long periods without food or water, Brook's body seems to be able to act as a normal person's. That is, he is either shown or implied to be able to eat, drink, cry and digest normally. His skeletal body may possibly give him enhanced resistance to pain or injury, as he would be more difficult to injure without blood or organs.